PHOSPHATE BUFFERS

IDENTIFICATION AND USE: Gomori buffers, the most commonly used phosphate buffers, consist of a mixture of monobasic dihydrogen phosphate and dibasic monohydrogen phosphate. By varying the amount of each salt, a range of buffers can be prepared that buffer well between pH 5.8 and pH 8.0.

Phosphates have a very high buffering capacity and are highly soluble in water. However, they have a number of potential disadvantages: Phosphates inhibit many enzymatic reactions and procedures that are the foundation of molecular cloning, including cleavage of DNA by many restriction enzymes, ligation of DNA, and bacterial transformation. Because phosphates precipitate in ethanol, it is not possible to precipitate DNA and RNA from buffers that contain significant quantities of phosphate ions.

FOUND IN THE FOLLOWING VACCINES: HEP B (ENGERIX-B), MENINAGOCOCCAL (MCV4-MENACTRA), HEP A+HEP B (TWINRIX), INFLUENZA (FLUZONE)