MODIFIED MUELLER

AND MILLER MEDIUM

IDENTIFICATION AND USE: the procedure involves the use of a variant and somewhat unstable strain of Clostridium Tetani and a culture medium containing a pancreatic digest of casein with additional cystine and tyrosine, beef heart infusion, glucose and inorganic salts. A high concentration of iron must be provided. The “modified” makes it more stable. Casein is a milk protein. Cystine and tyrosine are amino acids. Glucose is the sugar that cells burn. “Pancreatic digest” means that enzymes from the pancreas of an animal are used to break down the soup so the tetanus bacterium can eat it. A basic medium constituted as follows served this purpose: Pancreatic digest of casein, 150.0mg; glucose, 75.0mg; cysteine, 1.25mg; tyrosine, 2.5mg; NaCl, 25.0mg; Na2HPO4, 5.0mg; KH2PO4, 1.75mg; MgSO4.7H20, 0.5mg; reduced iron powder, 2.5 to 5.0mg; H2O to 10ml.

FOUND IN THE FOLLOWING VACCINES: HIB (ACTHIB), MENINGOCOCCAL (MCV4-MENACTRA), DTAP (DAPTACEL), DTAP + IPV + HIB (PENTACEL), TDAP(ADACEL)